FIG 5 .

Localization of Cda2 ω site mutants. (A) Schematic illustrating the domain organization of wild-type CDA2 and indicating the presence of the ω site. The cda2-ωΔ construct encodes a truncated version of Cda2 lacking the ω site and subsequent hydrophobic tail. In cda2-ωPM, the ω-1 through ω-5 sequence has been replaced with the corresponding sequence from S. cerevisiae Yps1. (B) Western blot analysis detecting Cda2 in fractions from KN99 (wild type [WT]) and the cda2Δ, cda1Δ cda2-ωΔ cda3Δ, and cda1 Δcda2-ωPM cda3Δ mutants. Cda2 in each fraction migrated at the same apparent molecular mass as shown in Fig. 1.