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. 1979 Oct 10;7(3):689–703. doi: 10.1093/nar/7.3.689

Analysis of RNA secondary structure by photochemical reversal of psoralen crosslinks.

D Rabin, D M Crothers
PMCID: PMC328048  PMID: 116192

Abstract

Aminomethyltrioxsalen (AMT), a psoralen, is known to cause interstrand crosslinks in double stranded nucleic acids. We have demonstrated the photochemical reversal of this reaction, and have used this result to develop a method for identification of specific sequences which are adjacent because of RNA secondary structure formation. E. coli 5S rRNA is used as a model system. We isolated and characterized a product that is derived from the stem region of 5S RNA.

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Selected References

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