Fig. 2.
Western blot analysis for Slit2/3 and Robo1/4 in OVCAR-3 and SKOV-3 cells. Cells at 80–90% confluence were harvested and subjected to Western blotting for Slit2/3 and Robo1/4. Different lanes in each individual ovarian cancer cell line represent different passages of cells. Positive controls for Slit2, Slit3, and Robo1/4 are human hepatoblastoma cell lysate, mouse thyroid extract, and HUVE cells, respectively