Table 1.

Derivation of the cell lines of the NCI-60 panel and results of virus screening.

Cell line name	Tumor type	Immuno-blotting for Env	PCR for enva	PCR for gaga	Passaged through miceb	Reference
BT-549	Breast	-	-	-	Nc
Hs-578T	Breast	-	-	-	N	[3]
MCF-7	Breast	-	-	-	N	[4]
MDA-MB-231	Breast	-	-	-	N	[5]
T-47D	Breast	-	-	-	N	[6]
MDA-MB-468	Breast	-	-	-	N	[7]
SF-268	CNS	-	-	-	N	[8]
SF-295	CNS	-	-	-	N	[8]
SF-539	CNS	-	-	-	N	[9]
SNB-19d	CNS	-	-	-	N	[10,11,12]
SNB-75	CNS	-	-	-	N	[10,11]
U251d	CNS	-	-	-	N	[12,13]
COLO-205	Colon	-	-	-	N	[14]
HCC-2998	Colon	-	-	-	Ye
HCT-116	Colon	-	-	-	N	[15]
HCT-15	Colon	-	-	-	N	[16]
HT29	Colon	-	-	-	N	[17]
KM12f	Colon	-	-	-	N	[18]
SW-620	Colon	-	-	-	N	[19]
CCRF-CEM	Leukemia	-	-	-	N	[20]
HL-60	Leukemia	-	-	-	N	[21]
K562	Leukemia	-	-	-	N	[22]
MOLT-4	Leukemia	-	-	-	N	[23]
RPMI-8226	Leukemia	-	-	-	N	[24]
SR	Leukemia	-	-	-	N	[25]
LOX IMVI	Melanoma	-	-	-	Y	[26,27]
M14g	Melanoma	-	-	-	N	[28,29]
MALME-3M	Melanoma	-	-	-	N	[17]
MDA-MB-435g	Melanoma	-	-	-	N	[7,29,30,31]
SK-MEL-2	Melanoma	-	-	-	N	[17]
SK-MEL-28	Melanoma	-	-	-	N	[32]
SK-MEL-5	Melanoma	-	-	-	N	[32]
UACC-257	Melanoma	-	-	-	Ne
UACC-62	Melanoma	-	-	-	Ne
A549 ATCC	Non-Small Cell Lung	-	-	-	N	[33]
EKVX	Non-Small Cell Lung	+	+	+	Y	[26]
HOP-62	Non-Small Cell Lung	-	-	-	Ye	[34,35]
HOP-92	Non-Small Cell Lung	-	-	-	Ne	[34,35]
NCI-H226	Non-Small Cell Lung	-	-	-	N	[36,37]
NCI-H23	Non-Small Cell Lung	-	-	-	N	[36,37]
NCI-H322M	Non-Small Cell Lung	-	-	-	N	[37,38,39]
NCI-H460	Non-Small Cell Lung	-	-	-	N	[36,37]
NCI-H522	Non-Small Cell Lung	-	-	-	N	[36,37]
IGR-OV1	Ovarian	-	-	-	N	[40]
NCI/ADR-RESh	Ovarian	-	-	-	N	[41,42,43]
OVCAR-3	Ovarian	-	-	-	N	[44]
OVCAR-4	Ovarian	-	-	-	N	[45]
OVCAR-5	Ovarian	-	-	-	N	[45]
OVCAR-8	Ovarian	-	-	-	N	[45,46]
SK-0V-3	Ovarian	-	-	-	N	[17]
DU145	Prostate	-	-	-	N	[47]
PC-3	Prostate	-	-	-	N	[48]
A498	Renal	-	-	-	N	[33]
ACHN	Renal	-	-	-	N	[49]
CAKI-1	Renal	-	-	-	N	[17]
RXF-393	Renal	-	-	-	Y	[50]
SN12C	Renal	-	-	-	N	[51]
TK-10	Renal	-	-	-	N	[52]
UO-31	Renal	-	-	-	Ui
786-0	Renal	-	-	-	N	[53]

^a “+”indicates a definitive band of the expected size was observed.

^b Y=Yes. N=No. U=Unknown; no information was available from literature references.

^c This cell line was obtained from ATCC. According to the ATCC description: “The BT-549 line was isolated in 1978 by W.G. Coutinho and E. Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes.” The establishment of other breast cancer cell lines by the same group has been described [54].

^d SNB-19 and U251 are derived from the same individual [12].

^e Michael Alley, NCI, personal communication.

^f The KM12 cell line is the same as KM12C in Morikawa et al. [18] (Michael Alley, NCI, personal communication).

^g Current samples of M14 and MDA-MB-435 are related [29]. MDA-MB-435 was originally isolated as a breast cancer cell line.

^h NCI/ADR-RES was originally described as a breast cancer cell line, but is related to the ovarian cancer cell line OVCAR-8 [41].

ⁱ Highly unlikely to have been passaged through mice (W. Marston Linehan, NCI, personal communication).