Table 1.
The effect of the increasing concentrations of SD on the relative contents of protein markers for cell proliferation and apoptosis in the untreated control B16F10 melanoma cells, and cells treated with 62.5, 125 and 250 µM concentrations of SD for 24 h and 72 h. Values are means ± SDV (standard deviation); n = no. of determinations and (*) values with statistical difference (p < 0.05) as compared to untreated control cells.
| Sarcophine-diol concentration (µM) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 62.5 | 125 | 250 | 0 | 62.5 | 125 | 250 | ||
| 24 h treatment | 72 h treatment | ||||||||
| Proliferation markers | STAT-3 | 100% | 78.8 ± 9.5 n = 4 | 62.9 ± 10.4 n = 4 (*) | 36.0 ± 6.0 n = 4 (*) | 100% | 70.4 ± 18.2 n = 3 | 59.8 ± 8.4 n = 3 (*) | 41.8 ± 10.5 n = 3 (*) |
| Cdk4 | 100% | 105.3 ± 9.2 n = 3 | 92.8 ± 14.3 n = 3 | 101.8 ± 15.3 n = 3 | 100% | 112.5 ± 27.2 n = 3 | 97.4 ± 18.9 n = 3 | 118.7 ± 20.1 n = 3 | |
| Cyclin-D1 | 100% | 75.3 ± 10.5 n = 3 | 35.5 ± 7.2 n = 3 (*) | 7.7 ± 14.2 n = 3 (*) | 100% | 87.8 ± 12.6 n = 3 | 48.9 ± 9.8 n = 3 (*) | 4.9 ± 6.2 n = 3 (*) | |
| Cyclin-D2 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Apoptosis markers | C-PARP | 100% | 123.2 ± 18.5 n = 3 | 132.1 ± 18.1 n = 3 (*) | 168.3 ± 24.1 n = 3 (*) | 100% | 189.3 ± 32.2 n = 3 (*) | 216.0 ± 42.7 n = 3 (*) | 276.0 ± 56.8 n = 3 (*) |
| p53 | 100% | 128.0 ± 23.9 n = 3 | 132.1 ± 19.6 n = 3 (*) | 132.7 ± 16.9 n = 3 (*) | 100% | 141.5 ± 33.3 n = 3 | 159.9 ± 22.6 n = 3 (*) | 177.6 ± 19.4 n = 3 (*) | |
| C-Casp-9 | 100% | 124.3 ± 27.7 n = 3 | 132.3 ± 20.5 n = 3 (*) | 147.4 ± 18.1 n = 3 (*) | 100% | 130.6 ± 23.5 n = 3 | 158.0 ± 31.6 n = 3 (*) | 179.1 ± 18.9 n = 3 (*) | |
| C-Casp-3 | 100% | 120.2 ± 14.3 n = 3 | 133.3 ± 9.9 n = 3 (*) | 140.3 ± 16.7 n = 3 (*) | 100% | 128.2 ± 25.5 n = 3 | 141.7 ± 22.5 n = 3 (*) | 174.2 ± 21.0 n = 3 (*) | |
| C-Casp-8 | 100% | 119.5 ± 22.6 n = 3 | 132.3 ± 25.3 n = 3 (*) | 160.3 ± 18.7 n = 3 (*) | 100% | 123.9 ± 25.1 n = 3 | 178.3 ± 33.6 n = 3 (*) | 213 ± 51.2 n = 3 (*) | |