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Analysis of the fractions, containing Suberitine, obtained during the purification procedure by SDS-PAGE (15% acrylamide/0.1% SDS); 3 µg of protein were applied per slot. (lane a) Fraction II obtained after ammonium sulfate fractionation; (lane b) Fraction III after gel chromatography on Sephadex G-100; (lane c) Fraction IV after a final gel filtration chromatography step (Sephadex G-50). The arrow marks the single protein band of an apparent size of 7000 to 8000: (M) Size markers.
