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. 2012 Jan 18;10(1):177–199. doi: 10.3390/md10010177

Figure 8.

Figure 8

Expression of the carotene dioxygenase and the carotenoid oxygenase 2 gene in primmorphs. The aggregates remained in the absence of or were incubated with 3 µM β-carotene. Three days after formation of the primmorphs the aggregates were exposed to blue, green or red light for an additional 3 days. Then the steady-state level of carotene dioxygenase and of carotenoid oxygenase 2 was determined by qPCR using tubulin as reference gene. The expression levels are shown of the two oxygenases of primmorphs kept either in the absence (−) or presence (+) of 3 µM β-carotene and exposed to light of different wavelengths. The increase of the carotene dioxygenase expression is significant (*P < 0.05 referred) in primmorphs if the light exposed cultures had been incubated also with β-carotene. The bars represent the means (±SD) from 10 separate experiments each.