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. 2011 Dec 9;31(4):1041–1053. doi: 10.1038/emboj.2011.454

Figure 7.

Figure 7

Effect of PulI residue substitutions on pilus assembly. (A) ETEC's GspI–GspJ–GspK complex showing residues E35, W42, E45 and N46 in GspI. (B) Two-hybrid assay of T25-PulJ chimera and WT T18-PulI or indicated mutant variants. Zip-T18 and Zip-T25 are used as positive control and T25 as negative. (C) Cysteine crosslinking of PulJL16C with PulIL10C or its indicated double-substituted variants. (D) Pilus assembly in strains co-producing PulJ or PulJ and PulK with WT PulI or its variants N46K and W42A/N46K. (E) PulG percentage in SF (mean+s.d.) from three independent experiments like the one in (E). (F) PulA immunodetection in cell extracts and supernatants (C, SN) of E. coli expressing all pul genes with a pulI deletion complemented with WT pulI or indicated mutant derivatives. Figure source data can be found in Supplementary data.