FIG. 1.
Morphological analysis of umbilical cord blood- and bone marrow-derived hMSCs. Representative images after one or 3 days in vitro culture of cells from one line of each hMSCs type. Cells were seeded at the density of 2,500 cells/cm2 in passages 3, 5 and 5 for BMMSC >50, BMMSC <18 and UCBMSC respectively. (Magnification×100) (A). Cell surface area of hMSC types after 1-day and 3-day culture. Data are mean±SD of 60 cells. ###P<0.001 of differences between BMMSC >50 and BMMSC <18 in Mann–Whitney test and P value of ***P<0.001 when compared with UCBMSC with Mann-Whitney test (B). Cell size in suspension determined by forward scattering intensity by flow cytometry (C). Results are mean±SD of 3 different cell lines. ##P<0.01 for differences between BMMSC >50 and BMMSC <18 in 2-sample t-test. **P<0.01 when compared with UCBMSC with 2-sample t-test. hMSC, human mesenchymal stem cell; UCBMSC, umbilical cord blood-derived mesenchymal stem cells; BMMSC, bone marrow-derived mesenchymal stem cells; SD, standard deviation.