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. 2011 May 26;21(4):575–588. doi: 10.1089/scd.2011.0023

FIG. 4.

FIG. 4.

Quantification of mitochondrial superoxide and MnSOD, in UCBMSC, BMMSC <18 and BMMSC >50. Percentage of MitoSOX positive cells. Results are mean±SD of 3 different hMSC lines (A). Measurement of MitoSOX intensity after rotenone treatment. Results are represented as mean±SD of 2 different hMSCs lines (B). Representative flow cytometry contour plots of MitoSOX stainings in undifferentiated UCBMSC, BMMSC <18, and BMMSC >50 (C, E, and G) and after 14 days of osteoinduction (D, F, and H). Representative western blot of MnSOD levels from 2 different hMSC lines and comparison of MnSOD intensities from 3 different hMSC lines. Fold changes are represented as mean±SD of 3 hMSC line (I). */# represent 2-sample t-test P values P<0.05. # indicates differences between control and rotenone treatment. MitoSOX positive gate was determined by negative controls individually. Numbers inside gates indicate percent of cells positive for MitoSOX. 14-day ost refers to 14-day osteoinduction. MnSOD, manganese superoxide dismutase.