FIG. 5.
Oxygen consumption after osteoinduction of UCBMSC and BMMSC. Complex II-mediated state 4 (Succinate) and state 3 (Succinate and ADP) oxygen consumption rates of UCBMSC and BMMSC. Results are represented as a mean±SD of 3 different lines (A). The state-4-mediated oxygen consumption rate was significantly higher in 14-day osteoinduction than in BMMSC <18, whereas no significant differences between BMMSC <18 and 14-day osteoinduced cells were seen in state 3 (B). 14-day osteoinduction also showed a significant increase in state 4 but not in state 3 oxygen consumptions when compared with UCBMSC (C). Results are represented as mean of 4 parallel measurements±SD (D). Oxygen consumption rate in undifferentiated cells decreased to zero after a short period of time without inhibitors; whereas in osteoinduced cells, the oxygen consumption was more continuous. *P<0.05 in Mann–Whitney test between control and osteoinduced cells. Oxygen consumption measurements were performed with 550,000 cells in all experiments. Glu+Mal, glutamate and malate; ADP, adenosine diphosphate; Rot, rotenone; Succ, succinate, ΔO2; difference in initial oxygen concentration between cell suspensions.