FIG. 6.

Mitochondrial membrane potential after short-term osteoinduction. Intensity of JC-1 aggregates (emission at 585 nm) measured by flow cytometry represented as mean±SD of 3 different hMSC lines (A). 585 nm/530 nm of JC-1 analysis represented as mean±SD of 3 parallel experiments from undifferentiated and 14-day osteoinduced cells (B). Representative flow cytometry contour plots of undifferentiated UCBMSC and BMMSC (C and E) and after 14 days of osteoinduction (D and F). Samples were stained with JC-1 and analyzed by flow cytometry. R1 means viable cells; R2 and R3, early apoptotic cells; and R4, late apoptotic cells. Numbers indicates percent of cells belonging to indicated group. **/## and *** indicates 2-sample t-test P values P<0.01 and P<0.001. In (A), * indicates differences when compared with UCBMSC and in (B), differences between undifferentiated and osteoinduced cells. # indicates differences between BMMSC <18 and BMMSC >50.