Table 2.
Mitochondrial and Cellular Properties Compared with Expression of Characteristics Cell Surface Antigens of Human Mesenchymal Stem Cells
| Mitochondrial and cellular properties | UCBMSC | BMMSC <18a | BMMSC >50b | 14-day osteoinduction |
|---|---|---|---|---|
| Proliferation rate | +++++ | ++++ | ++ | – |
| Mitochondrial area | ++++ | +++ | + | +++++ |
| Mitochondrial superoxide levels | +++++ | ++ | ++ | +++++ |
| Manganese superoxide dismutase levels | +++ | + | + | +++++ |
| Oxygen consumption | ++ | ++ | ++ | +++++ |
| Cell surface antigen expression | ||||
| CD90 | 78,16 (30,7) | 99,69 (0,2) | 99,77 (0,1) | – |
| CD105 | 99,52 (0,6) | 99,72 (0,4) | 99,69 (0,2) | – |
| CD73 | 99,89 (0,1) | 99,78 (0,3) | 99,42 (0,4) | – |
| CD44 | 99,70 (0,4) | 99,66 (0,2) | 99,40 (0,3) | – |
| CD49e | 99,79 (0,4) | 99,68 (0,2) | 98,95 (0,5) | – |
| HLA-ABC | 95,11 (3,3) | 99,60 (0,3) | 98,41 (0,8) | – |
| HLA-DR,CD19,CD45,CD34,CD14 | 6,82 (1,5) | 1,97 (0,1) | 0,87 (0,5) | – |
Cell surface antigen expressions are represented as mean±standard deviation from 3 different hMSC lines. Cells were stained with particular antigens and analyzed by flow cytometry. Positivity for antigens was defined by negative and isotype controls. +++++ indicates the highest value for cellular and mitochondrial properties. −indicates lack of analysis.
a
BMMSC <18, bone marrow mesenchymal stem cells from donors under 18 years old.
b
BMMSC >50, bone marrow mesenchymal stem cells from donors over 50 years old.
hMSC, human mesenchymal stem cell.