FIG. 2.

Overexpression of Ngn1, Ngn2, and Ngn3 in HFC7 cell population decreases the proportion of proliferating cells, whereas Mash1 does not affect proliferation. (A) Impact of proneural gene overexpression on proliferation, expressed as the relative quantity of Ki67⁺ proliferating cells in transduced populations (LacZ, Ngn1, Ngn2, Ngn3, and Mash1) reported on the quantity of Ki67⁺ proliferating cells in the control LacZ population. Proliferation underwent a highly significant decrease after Ngn1, Ngn2, and Ngn3 overexpression, whereas it was not affected by Mash1 overexpression, highlighting different roles of Ngns and Mash1 in hNPC proliferation. Student's t-test: ***P<0.001. (B) Immunofluorescent staining against Ki67 (green), illustrating hNPC proliferation in control populations (LacZ) and after overexpression of Ngn1, Ngn2, Ngn3, and Mash1. Scale bar: 50 μm.