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. 2011 May 11;21(4):539–553. doi: 10.1089/scd.2011.0079

FIG. 4.

FIG. 4.

Ngn2 overexpression affects both neuronal and astroglial pathways in HFC7 cells. (A) Proportions of HFC7 cells from different neural lineages at 4 days after transduction with the VSV-PGK-LacZ and VSV-PGK-Ngn2 vectors. Overexpression of Ngn2 did not affect the global proportions of Nestin+ cells but induced a highly significant increase in the proportions of GFAP+ astrocytes and β3 Tubulin+ neuroblasts, together with a significant increase in the proportions of A2B5+ cells, MAP5+ neuroblasts, and MAP2+ mature neurons. (B) Immunofluorescent staining against the various markers tested in A, after hNPC transduction with the VSV-PGK-LacZ (upper cases) and VSV-PGK-Ngn2 (lower cases) vectors. (C) The global lack of changes in the proportion of Nestin+ cells contrasted with the effects of Ngn2 overexpression on the neuroepithelial and astroglial cell populations. Overexpression of Ngn2 induced a very significant decrease in the proportion of Nestin+/GFAP− neuroepithelial cells, along with a highly significant increase in the proportion of Nestin+/GFAP+ astrocytes. (D) The increase in the proportion of A2B5+ cells reflected a very significant increase in the proportion of A2B5+/MAP5+ neuroblasts exclusively. Ngn1 overexpression did not affect the proportion of A2B5+/MAP5-OPCs. Student's t-test: *P<0.05; **P<0.01; ***P<0.001. Scale bar: 50 μm.