Figure 3.

Average daily alcohol intake (g/kg/day) during the first weeks of vapor-induced drinking in (a) Crhr1^NestinCre mice (n=11) and in their control counterpart mice (n=12), as well as in (b) CRH1R^−/− mice (n=7) and in their control counterpart mice (n=9). All mice were exposed to voluntary 8% home-cage drinking for 3 months. Thereafter they received four cycles of 16-h ethanol vapor exposure separated by 8-h periods of withdrawal. Twenty-four hours after discontinuation of vapor exposure, all mice (except one CRH1R^−/− mouse that died during the vapor exposure) were returned to voluntary home-cage drinking for 4 weeks. Alcohol intake measured during the last week before vapor exposure is marked as baseline ‘B'. Data are presented as means±SEM ^*indicates significant differences from the control mice group after post-hoc analysis (p<0.05). Although we report in the Results section significant genotype differences between Crhr1^NestinCre and their control mice line during post-dependent drinking, we did not find significant interactions by time and therefore do not show post-hoc analyses here.