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. 2011 Nov 16;37(4):1005–1012. doi: 10.1038/npp.2011.284

Figure 1.

Figure 1

Expression of R7 family members and interacting proteins in the mouse brain. (a) The expression of R7BP, Gβ5, RGS9-2, and RGS6/7 and in different brain regions of the mouse. Western blot analysis was also used to determine possible regulation of R7BP levels in the mouse striatum by acute (30 min or 2 h, n=4–5 per group) or repeated morphine application (once a day for 4 days, n=4 per group). As shown in (b), acute or repeated morphine treatments do not affect R7BP levels. Co-immunoprecipitation assays in the striatum demonstrate an increase in RGS9-2/R7BP complexes following acute fentanyl or morphine administration (c; n=3 per group). Striata from C57/Bl/6 mice treated with saline (sal) fentanyl (fent) or morphine (mor) for 30 min were immunoprecipitated with anti-RGS9-2 antibody and assayed with anti-R7BP antibody. Total lysates (TL) were assayed for R7BP levels. Data are expressed as mean±SEM, n=3 per group *p<0.05, one-way ANOVA followed by Dunnett's post hoc test.