Figure 6.

R7BP does not affect morphine withdrawal. Mice received increasing morphine doses as described in Methods and withdrawal was precipitated using naloxone hydrochloride (1 mg/kg). Several withdrawal signs (jumps, wet dog shakes, tremor, diarrhea, and weight loss) were monitored for 30 min after naloxone administration. No significant difference was observed between genotypes, although there was a trend for decreased diarrhea and weigh loss in mutant mice. Data are expressed as mean±SEM, n=12–14 per group.