Figure 2.

Evaluating the role of biotinylation extent or linker length. Various biotinylated llama anti-ricin IgG were examined in a direct binding assay to ricin A chain-coated microspheres; Panel A shows signal with increasing number of biotins per antibody (D/P) as determined using sureLINK biotin, while Panel B shows labeling with Lc and Lc-Lc biotin at different ratios of biotin-NHS to antibody. Results indicated that high biotin to protein ratios and longer linker lengths produce superior reagents.