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. 2009 Jan 19;9(1):542–555. doi: 10.3390/s90100542

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — ELISA based ricin detection. Phage-displayed sdAb and llama polyclonal anti-ricin IgG were compared as the reporter reagents using a llama anti-ricin capture surface. Panel A shows results of phage-displayed sdAb followed with an anti-M13 –HRP conjugate for color generation and biotinylated llama anti-ricin IgG followed with a Streptavidin – HRP conjugate. Panel B show the results using biotinylated phage-displayed sdAb and biotinylated soluble sdAb reporter elements followed with a Streptavidin –HRP conjugate. Data is plotted as the average of 3 wells, with error bars representing the standard deviation. Results indicate phage-displayed sdAb can act as highly sensitive detection reagents.