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. 2009 Jan 19;9(1):542–555. doi: 10.3390/s90100542

Figure 6.

Figure 6.

Dot blot assay for ricin, ricin A chain, and ricin B chain using C8-phage (left) and C8-sdAb (right). C8-phage binding was visualized using HRP labeled anti M13 IgG while the C8-sdAb was visualized using HRP labeled anti-His. Color formation was generated using sigmafast Diaminobenzidine (DAB). 5 or 2 μL of ricin, ricinA, ricin B or BSA (10 μg/ mL) were spotted onto nitrocellulose as the target for both phage-displayed and soluble sdAb.