TABLE 1.
Taqman assays forHIV-1 plasmid of different subtypes/CRFs
| Subtype/CRFs | NIH catalog No. | Reagent | CT valuea | HIV-1 Copies.b |
|---|---|---|---|---|
| A | 3605 | p92UG037.1 | 32 ± 1.2 | 1×103 |
| B | 114 | pNL4-3 | 32 ± 0.8 | 1×103 |
| B | 11414 | pWT/Bal | 32 ± 1.3 | 1×103 |
| B/C | 2003 | pSG3.1 | 33 ± 1.5 | 1×103 |
| C | 6187 | P98TZ013.10 | 34 ± 1.9 | 1×103 |
| D | 4001 | p94UG114.1 | 34 ± 1.8 | 1×103 |
| A/E | 4007 | p90CF402.1.8 | 33 ± 1.8 | 1×103 |
| F | 4004 | p93BR020.1 | 32 ± 1.2 | 1×103 |
| H | 4005 | p90CF056.1 | 32 ± 1.3 | 1×103 |
a
CT shown was the mean ± SD from 3 independent assays each representing the mean of results from triplicate wells (n=9).
b
HIV-1 Copy number was calculated based on plasmid size and the concentration of the plasmid by UV spectrophotometry.