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Gpx3 protein in plasma and kidney from mice of differing selenium nutritional status. Plasma samples and kidney homogenates were subjected to SDS/PAGE, and Western blotting was carried out. Protein (50 μg) in the form of 10% kidney homogenate or 0.5 μl plasma was loaded per lane. Density of the Gpx3 band was determined and compared with the average density from mice fed 0.25 mg selenium/kg diet. Values are means ± SD, n = 5.
