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. 2012 Feb 13;209(2):225–233. doi: 10.1084/jem.20111044

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© 2012 Gehrig et al.

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Figure 3. — Molecular surgery approach based on catalytic DNA. (A and B) A DNAzyme was engineered to cut between residues 24 and 25 of tRNA^Tyr (85 nt), generating fragments of 61 nt and 24 nt. (C) Fragments of cleaved native tRNA^Tyr₁ and its IVT were purified by PAGE (indicated by arrows). (D) Isolated unmodified (black lines) and modified fragments (gray lines with dots indicating modifications) were annealed on splint cDNA to yield modivariants mv#1 and mv#2 after ligation and digestion of cDNA. (E) PAGE purification of modivariants (indicated by arrows).