Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Feb 13;209(2):275–289. doi: 10.1084/jem.20111117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Schulz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Depletion of Hsp90, HDAC6, or Hsf1 all destabilize MIF protein. (A and B) MDA231 and 5637 cells were transfected with siRNA against the Hsp90 chaperone (A) or against HDAC6 (two different sequences, siHDAC6_1 and siHDAC6_2; B). After 3 d, MIF and Hsp90 protein levels were assessed by immunoblots. Representative blots from two independent experiments are shown. Actin, loading control. (C and D) 5637 and U2OS cells transfected with two different siRNAs against Hsf1 for 3 d (C), and MDA231 cells stably transfected with an shRNA against Hsf1 were immunoblotted for MIF, Hsp90, Hsp70, and Hsf1 (D). Representative blots from three independent experiments. Actin, loading control. (E) Untreated HCT116 cells were subjected to coimmunoprecipitation with anti-MIF or irrelevant anti-HA antibodies and immunoblotted with isoform-specific Hsp90 antibodies.