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. 2012 Feb 13;209(2):407–421. doi: 10.1084/jem.20111493

Figure 2.

Figure 2.

Leukocyte rolling and adhesion in kidney or cremaster venules after ischemia-reperfusion is dependent on SLP-76 and ADAP. (A and B) Untreated LysM-GFP mice or LysM-GFP mice pretreated with different antibodies (anti–Mac-1 antibody [M1-70], n = 3; anti–LFA-1 antibody [TIB217], n = 3; and anti–E-selectin antibody [9A9], n = 3) were subjected to RIR, and the rolling velocity (A) and the number of adherent leukocytes (B) in venules of the kidney were determined. (C) Representative pictures of cremaster muscle postcapillary venules of untreated LysM-GFP mice and LysM-GFP mice pretreated with a blocking anti–E-selectin antibody (Ab) 4 h after renal IRI. Bars, 25 µm. (D and E) 2 h after RIR, WT mice were injected i.v. with fluorescently labeled BM cells from WT (n = 3), Slp76−/− (n = 3), Slp76Y145 (SM; n = 3), Slp76Y112/128 (DM; n = 3) or ADAP−/− (n = 3) mice. 2 h later, leukocyte rolling velocity (D) and the number of adherent cells (E) in venules of the kidney were determined. (F and G) The cremaster muscle of WT (n = 3), Slp76−/− (n = 3), Slp76Y145 (n = 3), Slp76Y112/128 (n = 3), and ADAP−/− (n = 3) mice was subjected to ischemia (30 min)/reperfusion (120 min) injury, and mean rolling velocity (F) and the number of adherent cells (G) in postcapillary venules of the cremaster muscle were determined. Results are presented as means ± SEM. #, P < 0.05.