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. 2012 Feb 13;209(2):251–258. doi: 10.1084/jem.20111703

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© 2012 Shaw et al.

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Figure 3. — IL-1R signaling on T cells is required for the generation of intestinal sT_H17 response. (A) Bone marrow chimeras were generated by reconstituting lethally irradiating SPF wild-type or Ilr1^−/− recipients with 10⁶ donor bone marrow cells isolated from the indicated mice. Intestinal sT_H17 response was assessed at 10 wk after reconstitution. Production of IL-17, IL-22, and IFN-γ by (10⁶ cells/ml) total LP cells was assessed ex vivo in the indicated chimera mice after stimulation with (soluble, 1 µg/ml) anti-CD3. The results are shown from one of two independent experiments with five chimera mice per group per experiment. (B, left) Experimental procedure for adoptive T cell transfer. (B, right) Representative FACS dot plot analysis of the frequency of CD4⁺Rorγt-gfp⁺ cells in various organs at day 14 after adoptive transfer. (C) Production of IL-17 and IFN-γ by (10⁶ cells/ml) total LP cells isolated from adoptively transferred mice (B, right) after ex vivo stimulation with anti-CD3. Data shown are from one representative experiment of three independent experiments with five chimeric mice per group per experiment.