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. 2011 Nov 15;12(10):872–880. doi: 10.4161/cbt.12.10.17672

Figure 2.

Figure 2.

VEGFR-1 shRNA1 and shRNA2 efficiently knocked down VEGFR-1 expression at both mRNA and protein levels in 786-O cells. (A) RT-PCR analysis of VEGFR-1 expression in parental 786-O cells and 786-O cells stably transfected with VEGFR-1 shRNA1, shRNA2, shRNA3, and control shRNA. (B) Densitometry quantification of the RT-PCR results in (A). (C) Protein gel blot analysis of VEGFR-1 expression in parental 786-O cells and 786-O cells stably transfected with either shRNA1 or control shRNA. Protein gel blot of β-actin is included as a loading control. (D) Densitometry quantification of the protein gel blot results in (C). (E) RT-PCR analysis of VEGFR-1 and VEGFR-2 mRNA expression in 786-O cells transfected with control shRNA, VEGFR-1 shRNA1, and shRNA2. RT-PCR of 28S mRNA was included as an internal control. (F) Densitometry quantification of the RT-PCR results in (E).

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