Figure 5.

VEGFR-1 knockdown inhibits MCP-1 expression and macrophage infiltration. (A) Representative pictures of immunohistochemical staining of control and VEGFR-1 knockdown tumor sections with antibody against F4/80, a marker of macrophages. (B) The macrophage count was significantly decreased in VEGFR-1 knockdown tumors compared with control tumors. *p < 0.01 vs. control. (C) Representative pictures of MCP-1 RT-PCR in cultured control and VEGFR-1 knockdown 786-O cells, as well as in control and VEGFR-1 knockdown tumors. (D) The level of MCP-1 mRNA was significantly decreased in VEGFR-1 knockdown tumors compared with control tumors. *p < 0.01 vs. control tumors.