Figure 4.
Influence of IFNγ application on the generation of virus neutralizing antibodies. (A) Serum and ascitic fluid were collected from all groups of virus-treated rats (Fig. 2) and the titers of virus neutralizing antibodies (αH-1PV) were determined using cytotoxicity protection assay on NB324K cells. The titers are expressed as the percentage of antivirus protection offered by serum or ascitis dilutions compared with mock infected cells. (B) Two groups of metastasis bearing rats were treated with two intraperitoneal injections of H-1PV (3 × 108 pfu/injection per animal) spanning four weeks between them, with or without intermediate recIFNγ i.p. inoculation. Titers of αH-1PV in ascitic fluids were determined 10–30 d after the second H-1PV i.p. injection and expressed as indicated above.