Figure 5.

Release of TNFα from PDAC and PBMC cocultures after H-1PV+IFNγ treatment.The indicated pancreatic cancer cell lines were seeded into 10 cm² dishes at 1.5 × 10⁶ cells/ dish and infected or not with H-1PV at an MOI of 10 pfu/cell. Twenty-four hpi cells were harvested and plated onto pre-isolated PBMCs in 48 well plates at a ratio of PDAC:PBMC 1:5. The cocultures were treated or not with 50 UI/ml of human recombinant IFNγ and the release of TNFα was measured in supernatants 24 h later by ELISA. Mock or H-1PV infected (MOI 10) monocultures of PBMCs served as controls. The indicated values are average of at least three independent experiments. SD values are shown.