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. 2011 Nov 15;12(10):924–938. doi: 10.4161/cbt.12.10.17780

Figure 5.

Figure 5.

Tamoxifen induces TIGAR upregulation in cocultured MCF7 cells. (A) Fibroblast-MCF7 cell cocultures were cultured for 1 d with 12 µM tamoxifen or with vehicle alone. Then, cells were fixed and immuno-stained with anti-TIGAR (red) and anti K8–18 (green) antibodies. Nuclei were counterstained with DAPI (blue). TIGAR staining (red only) is shown in the top panels to better appreciate the tamoxifen-induced TIGAR upregulation in MCF7 cells. Original magnification 40x. (B) TIGAR is highly expressed in human epithelial breast cancer samples with loss of stromal Cav-1. Paraffin-embedded tissue sections from human breast cancer samples lacking Cav-1 were immunostained with antibodies directed against TIGAR. Slides were counterstained with hematoxylin. Note that TIGAR is highly expressed preferentially in epithelial breast cancer cells while stromal cells lack TIGAR expression. Original magnification, 40x.