Figure 2.

U0126 induces mitochondrial fluorescence. (A) Fluorescence imaging of LNCaP cells treated with U0126 for 12 h and stained with the mitochondrial dye Mitotracker CMXRos. Note the FITC perinuclear signal that colocalizes with the mitochondrial stain. (B) Fluorescence localization in the mitochondria is confirmed by cell fractionation and fractionation purity is assayed by protein gel blotting of compartment specific markers: α-tubulin for cytoplasm (cyto), MnSOD for mitochondria (mito), and lamin A/C for nucleus (nuc). (C) Multiple MEK inhibitors did not produce a fluorescent signal when incubated in C4–2 cells for 12 h. (D) U0126 produced fluorescence in multiple mammalian cells after 12 h incubation and (E) in BY4741 yeast.