Figure 5.

Role of glutamine metabolism in resveratrol induced apoptosis. (A) Transaminase inhibitors AOA and cycloserine were used in serum-free conditions. Twelve hours later resveratrol was added and cell death was assayed at 24 h by cleaved PARP or (B) nucleosome ELISA. **p < 0.05, paired two tailed t-test. (C) Cell death induced by resveratrol was assayed at varying glutamine concentrations. (D) Cell viability by crystal violet staining 60 h after resveratrol treatment at varying glutamine concentrations. **p < 0.05, paired one tailed t-test (E) The non-metabolizable glutamine analog, 6-diazo-5-oxo-L-norleucine (DON), did not restore resveratrol-induced death in glutamine-free medium.