Table 1. Sequence of shRNA.
Primer name | Sequence | mRNA target sequence | Position of mRNA |
---|---|---|---|
NC |
Sense:5′-CAACGACTTCATAAGGCGCATGCTTCAA GACGGCATGCGCCTTATGAAGTCTTTTTTG-3′ Antisense:5′-AGCTCAAAAAAGACTTCATAAGGCGC ATGCTTCAAGACGGCATGCGCCTTATGAAGTC-3′ |
GACTTCATAAGGCGCATGC |
Non-sense |
pYr-1.1A |
Sense:5′-CACCCTGCTGCCGCTGCTGCTACCGCTCGAGG ACGACGGCGACGACGATGGCTTTTTTG-3′ Antisense:5′-AGCTCAAAAAACTGCTGCCGCTGCTGCTAC CGCTCGAG GACGACGGCGACGACGATGGC-3′ |
CTGCTGCCGCTGCTGCTACCG |
904–924bp |
pYr-1.1B | Sense:5′-CACCCAAGCAGAGTACACACAGCATCTC GAGATGCTGTGTGTACTCTGCTTGTTTTTTG-3′ Antisense:5′-AGCTCAAAAAACAAGCAGAGTACACA CAGCATCTCGAGATGCTGTGTGTACTCTGCTTG-3′ |
CAAGCAGAGTACACACAGCAT | 1254–1274bp |