Table 2.
Differentially carbonylated proteins in TRX2 gene modified strains along biomass propagation process.
| Protein name | Time 0 h | Time 15 h | Time 80 h | |||
|---|---|---|---|---|---|---|
| TTRX2 vs T73 | trx2 vs T73 | TTRX2 vs T73 | Trx2 vs T73 | TTRX2 vs T73 | Trx2 vs T73 | |
| Oxidative stress and mitochondrial metabolism | ||||||
| Ilv5* | Nv | Nv | 0.52 (↓) | 0.38 (↓↓) | 0.45 (↓) | Nv |
| Ahp1p | Nv | Nv | 0.23 (↓↓) | 0.26 (↓↓) | Nv | Nv |
| Heat shock proteins | ||||||
| Ssa1p | Nv | 1.98 (↑) | 0.75 | 0.52 | 0.75 | Nv |
| Mif4p* | 0.50 (↓) | 2.93 (↑) | 0.57 (↓) | 0.49 (↓) | 0.36 (↓) | Nv |
| Ssb1p* | Nv | Nv | 0.86 | 2.38 (↑) | 1.43 | 2.37 (↑) |
| Sse1p* | 0.81 | 1.23 | 0.22 (↓↓) | 0.87 | 1.38 | Nv |
| Cpr5p | Nv | Nv | Nv | Nv | 0.82 | 0.85 |
| ATP Metabolism | ||||||
| Vam1p* | 0.92 | 3.05 (↑↑) | 0.15 (↓↓) | 0.54 (↓) | 0.53 (↓) | 0.69 |
| Ipp1p* | Nv | Nv | 0.65 | 2.24 (↑) | Nv | Nv |
| Atp2p* | Nv | Nv | 0.51 (↓) | 3.86 (↑↑) | 0.37 (↓) | Nv |
| Mam33p* | Nv | Nv | 0.48 (↓) | 2.13 (↑) | 0.86 | 1.72 (↑) |
| Tricarboxilic acid cycle | ||||||
| Mls1p* | Nv | 2.03 (↑) | 0.75 | 1.79 (↑) | 0.11 (↓↓) | 0.88 |
| Mdh1p* | Nv | Nv | 1.68 (↑) | 2.01 (↑) | Nv | 2.67 (↑) |
| Aco1p* | Nv | 1.53 (↑) | 0.86 | 4.03 (↑↑) | 0.76 | 1.91 (↑) |
| Glycolysis and Fermentation | ||||||
| Tdh3p* | 0.81 | 0.75 | 0.71 | 0.62 | 0.24 (↓↓) | 2.38 () |
| Eno1p* | Nv | Nv | Nv | 0.24 (↓↓) | 0.32 (↓) | 3.61 (↑↑) |
| Adh1p* | Nv | Nv | 0.95 | 0.98 | 0.13 (↓↓) | Nv |
| Pdc1p | Nv | Nv | 0.56 (↓) | 0.45 (↓) | 0.65 | 0.64 |
| Eno2p* | 0.46 (↓) | 0.53 (↓) | 0.85 | 0.52 (↓) | 0.29 (↓) | Nv |
| Fba1p* | Nv | Nv | 0.52 (↓) | 2.27 (↑) | 0.18 (↓↓) | 4.07 (↑↑) |
| Cdc19p* | Nv | Nv | 0.79 | 0.60 | 2.76 (↑) | Nv |
| Adl4p | Nv | Nv | 0.21 (↓↓) | 0.15 (↓↓) | 3.59 (↑↑) | 4.28 (↑↑) |
| Hxk1p* | Nv | Nv | 0.33 (↓) | 2.01 (↑) | 0.20 (↓↓) | 0.27 (↓) |
| Gpp1p | Nv | Nv | 0.53 (↓) | 0.41 (↓) | 0.85 | Nv |
| Tpi1p | Nv | Nv | Nv | Nv | 0.85 | 0.84 |
| Tdh1p* | 1.53 (↑) | Nv | Nv | Nv | 1.84 (↑) | Nv |
| Protein synthesis | ||||||
| Tef1p | 0.28 (↓↓) | 1.92 (↑) | Nv | Nv | 1.82 (↑) | 0.12 (↓↓) |
| Met6p | Nv | Nv | Nv | Nv | Nv | 0.85 |
| Rps5p* | Nv | Nv | Nv | Nv | Nv | 4.31 (↑↑) |
Represented data correspond to normalized Carbonyl intensity (CI) values from Western 2-D anti-DNP and Protein intensity (PI) from 2-D geles (Additional file 2) between the modified strain and control strains respectively. Ratios in bold character represent proteins with differential carbonylation pattern among TTRX2 and trx2 strains.
(↑) ratio [1.5-3]. (↑↑) ratio [ > 3], (↓) [0.5-0.25]. (↓↓) ratio [ < 0.25]
Nv: No variation respect to carbonylation levels in T73 control strain.
* Putative targets of Trx2p