Figure 2. Hpa effector ATR39-1 is recognized by A. thaliana ecotype Wei-0.
A) ATR39-1, but not ATR39-2, triggers a hypersensitive response (HR) in the Arabidopsis ecotype Weiningen (Wei-0) when delivered by Pf0. The top half of the leaf was inoculated with the indicated effector, the bottom half with a negative GFP control. Wei-0 also contains a functional allele of RPS2 and AvrRpt2 was included as a positive control for HR. The picture was taken 24 hours post infection. Stars highlight induction of HR. B) ATR39-1 recognition inhibits growth of virulent Pst DC3000 in a Col-0 line where the RPP39 locus was introgressed (WC 5BX), while Wei-0 displays natural resistance towards Pst DC3000. Plants were inoculated with Pst DC3000 expressing the indicated effectors (105 cfu/mL) and bacterial titers determined 0 and 4 days post-infection. Error bars indicate standard deviation. Significant differences as determined by Student's t-test are indicated by small letters (P<0.0003). Experiments were repeated at least twice with similar results.