Figure 4. The concentration of assembly intermediates for three initial protein concentration scenarios:

1) [vp2]₀ = 1.2 M, [vp6]₀ = 7.8 M and [vp7]₀ = 7.8 M (stoichiometric ratios – white bars); 2) [vp2]₀ = 1.2 M, [vp6]₀ = 7.8 M and [vp7]₀ = 0.78 M (limitation of vp7 – black bars); 3) [vp2]₀ = 12 M, [vp6]₀ = 7.8 M and [vp7]₀ = 7.8 M (excess of vp2 – grey bars). The Gibbs free energy of vp2, vp6 and vp7 subunit association was assumed to be equal (ΔG⁰_vp2,3-mer = ΔG⁰_vp6,13-mer = ΔG⁰_vp7,13-mer = −4.08 kcal.mol⁻¹).