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. 2012 Feb 16;8(2):e1002530. doi: 10.1371/journal.ppat.1002530

Figure 5. Gαq-Rho GEF Trio-Rho Signaling is required for aversion to pathogenic M. nematophilum.

Figure 5

Animals were placed equidistant from a two lawns of bacteria (A vs B) and the number of animals on lawns A and B were counted at 30 minutes (solid bars) and at 4 hours (hatched bars). The preference ratio shown is given by the formula [animals at A- animals at B/animals (A+B)]. Wildtype animals have no preference between E. coli (OP50) and pathogenic M. nematophilum at 30 minutes, but at 4 hours they have a strong preference for OP50 E. coli. This preference is abolished if the strain of M. nematophilum is avirulent (UV336) or if animals have a mutation in unc-73 or egl-30. Expression of EGL-30 in the motorneurons (MN::EGL-30) or of UNC-73 in all neurons (N::UNC-73) rescued the preference for OP50 in egl-30 and unc-73 mutants respectively. P values between 0.05 and 0.001 (*), and P values of 0.001 or less (**).