Skip to main content
. 2012 Feb 16;7(2):e31723. doi: 10.1371/journal.pone.0031723

Figure 2. Specific detection of EGFRvIII mRNA by real-time RT-PCR.

Figure 2

A. Real-time amplification plot of EGFRvIII transcript as detected in the U87MGvIII cell-line. U87MGvIII cells were used as a positive control, U87MG cells were used as negative control: water was used for no template control. B. Direct sequencing of U87MG and U87MGvIII cDNA confirms the presence of EGFRvIII transcript in U87MGvIII cells only. The broken arrow indicates the EGFRvIII-specific exon 1 and exon 8 junction. C. Linear dynamic range of the EGFRvIII real-time RT-PCR assay (100, 10, 1, 0.1 and 0.01 ng cDNA). D. EGFRvIII real-time RT-PCR assay amplification efficiency.