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TUNEL and TRAP double staining on ZA-treated tibial (A and B) and mandibular (C–E) fractures at day 5 post-injury. High magnifications (B, D, E) indicate apoptotic cells (black arrows) and TRAP-positive cells (white arrows) within the fracture callus. No TRAP-positive apoptotic cells can be detected at the fracture site. (A, C) Scale bar = 50 µm; (B, D, E) Scale bar = 10 µm.
