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. 2012 Feb 16;7(2):e31712. doi: 10.1371/journal.pone.0031712

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Spencer et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Human kidney labeled for HD5 (green), nuclei (blue) and nephron specific markers (red). Segment markers consisted of AQP-2 for collecting tubules (CT), THP for the loop of Henle (LOH), and AQP-1 for proximal tubules (PT). A/B: HD5 (green) was produced in the collecting tubules (red apical AQP-2 staining) of non-infected kidney tissue (A) and with pyelonephritis (B). Arrows indicate HD5 is produced in other nephron segments. C/D: HD5 (green) was expressed in the loops of Henle (red THP staining) in non-infected kidney tissue (C) and with pyelonephritis (D). E/F: HD5 (green) shows minimal production in the proximal tubules (red AQP-1 staining) of non-infected kidney tissue (E) and with pyelonephritis (F). Magnification 40×.