Figure 6. Differential expression of seven putative lipase genes at different developed stages.

PGs were collected at different developmental stages (−72, 0, and 72 h), and total RNA was extracted for real-time PCR analysis. The Rp49 gene was used as the housekeeping gene for normalization. The data represent the mean values ± SE of three biological replicates. Significance of pairwise comparisons (−72 h vs 0 h and −72 h vs 72 h) are marked with *** (p<0.001) as determined by the Student's t-test .