Figure 7. Effects of RNAi treatment on bombykol production.
A: RNAi-induced reduction of seven putative lipase genes. RT-PCR was carried out using cDNA generated from the total RNA extracted from PGs of females injected with 20 µg dsRNAs for seven lipase genes and DEPC-treated nuclease-free water (Control). B: Effects of RNAi for seven lipase genes on bombykol production. Newly emerged females were decapitated and injected with double stranded RNAs. Decapitated females were injected with 5 pmol PBAN 30 h after dsRNA injection. Bombykol production was measured by GC/MS from PGs 90 min after injection of PBAN. Bars indicate the mean values ± S.D. for independent experimental animals (n> = 6). Statistically significant differences from the PBAN alone are denoted by *** (p<0.001) as determined by the Student's t-test .
