Figure 2. Effects of morphine on HIV p24 protein or SIV p28 protein expression in macrophages.

Seven-day-cultured macrophages were treated with or without morphine (10⁻¹⁰ M) for 24 h and then incubated with HIV Bal strain or SIV Delta_B670 strain for 2 h in the presence or absence of morphine (10⁻¹⁰ M). HIV p24 (A) or SIV p28 (B) protein expression in macrophages at day 15 postinfection was determined by immunofluoresence staining with antibodies against HIV p24 or SIV p28 protein (green). The nuclei were stained with Hoechst 33342 (blue) (magnification, 100×; scale bar: 100 µm).