Table 2. Pma1 activity in situ (nmol Pi/min/mg total cell protein, n = 3±SD) after 15-min incubation of S. cerevisiae whole cells with 100 mM glucose or 100 mM deoxyglucose. The change of activity in % of initial activity is given in parenthesis.
Strain | Buffer | 100 mM glucose | 100 mM deoxyglucose |
BY 4742 | 7.36±2.94 (100) | 14.64±1.16 (198.94) | 5.64±1.54 (76.6) |
erg6 | 22.47±0.35 (100) | 44.0±0.1 (195.85) | 24.8±0.35 (110.38) |
RH 2874 | 29.62±0.75 (100) | 38.68±1.74 (130.59) | 21.27±0.36 (71.81) |
lcb1-100 | 38.34±0.96 (100) | 35.73±2.54 (93.2) | 36.18±0.13 (94.35) |
SEY 6210 | 18.5±0.89 (100) | 52.36±0.85 (283.1) | 16.8±2.19 (91) |
PMA1-GFP | 7.27±1.68 (100) | 23.19±0.22 (319.1) | 5.59±0.98 (77) |