Figure 5. While Arm protein levels are similar in larval imaginal tissues and stage 9 embryos, arm mRNA is more abundant in stage 9 embryos.

A. Immunoblot of cell extracts from stage 9 or stage 17 embryos, or from 3^rd instar brains and attached imaginal discs. Note that Arm is differentially spliced in neurons to produce a shorter form, neural Arm (nArm; [46]), which is present in stage 17 embryos and in the brain. B. Arm protein in larval imaginal tissues is at levels similar to those in stage 9 embryos, when normalized to tubulin as a loading control (the two tissues were not significantly different when compared by a one sample t test; p = 0.34). C. Northern blot of total RNA from stage 9 and stage 17 embryos and from 3^rd instar brains and attached imaginal discs. Densitometry revealed that arm mRNA is present at about 2 fold the level in stage 9 embryos than it is in larval imaginal tissues, when normalized to the ribosomal protein mRNA rp49.