Table 1. Immunization Composition and Regimen.

Animal	Group	Immunogens	LPSa	IFAb	Immunization Delivery Time (Week) and
		Multimeric Protein	DNA			Composition: P = Protein; D = DNA
						0	2	4	10	12	20	22	26	32
Rabbit c	1	Env(minV3)-E2+Env(V3)-E2	Env gp160	+	+	P		P	P			P+D	P+D	P+D
	2	Env(minV3)-E2+Env(V3)-E2	Env gp160	+	+	D		D		D	P		P
	3	Env(minV3)-E2+Env(V3)-E2	Env gp160	+	+	P+D		P+D		P+D	P+D		P+D
	4	Env(minV3)-E2	Env gp160	+	+	P+D		P+D		P+D	P+D		P+D
	5	Env(V3)-E2	Env gp160	+	+	P+D		P+D		P+D	P+D		P+D
	6	Env(V3)-E2	Env gp160	−	+	P+D		P+D		P+D	P+D
	7	Env(V3)-E2	Env gp160	−	−	P+D		P+D		P+D	P+D
	8	Env(V3)-E2	Env gp160	+	+	P+D		P+D				P+D	P+D
Mouse d	9	None	Env gp160	−	−	D	D
	10	Env(V3)-E2	None	+	−	P	P
	11	Env(V3)-E2	Env gp160	+	−	P+D	P+D
	12	Env(V3)-E2	Env gp160	−	−	P+D	P+D
	13	Unimmunized	None	−	−	−	−

^aLPS, lipopolysaccharide.

^bIFA, Incomplete Freund's adjuvant.

^c200 µg of E2 intramuscularly (IM) per animal per immunization; 36 µg of codon-optimized HIV-1 SF162 DNA intradermally via Gene Gun.

^d130 µg of E2 delivered IM; 500 µg (first dose) or 193 µg (second dose) of codon-optimized HIV-1 SF162 DNA was delivered IM.

In all cases blood samples were collected two weeks after each immunization.