Table 2. HIV-SF162 neutralization competition assay for V3 specificity.
ID50 in TZM-bl Assay1 (HIV-SF162) | ||||||
Group | Rabbit ID | Bleed week | No peptide | Scrambled V3 peptide | V3 peptide | Percent inhibition |
1 | 3045 | 34 | 118 | 118 | 40 | 67 |
2 | 6869 | 14 | 126 | 222 | 17 | 92 |
28 | 634 | 634 | 8 | 99 | ||
3 | 6864 | 6 | 714 | 1093 | 8 | 99 |
28 | 1058 | 963 | 29 | 97 | ||
6865 | 6 | 166 | 204 | 8 | 96 | |
28 | 519 | 660 | 43 | 93 | ||
6866 | 6 | 519 | 820 | 8 | 99 | |
28 | 251 | 206 | 60 | 71 | ||
4 | 6860 | 14 | 674 | 1143 | 143 | 87 |
28 | 568 | 669 | 66 | 90 | ||
6861 | 14 | 323 | 647 | 42 | 94 | |
28 | 420 | 251 | 25 | 90 | ||
6862 | 14 | 164 | 215 | 52 | 76 | |
28 | 442 | 327 | 67 | 80 | ||
5 | 6858 | 6 | 346 | 734 | 8 | 99 |
28 | 685 | 372 | 42 | 89 | ||
6859 | 6 | 180 | 314 | 8 | 97 | |
28 | 709 | 362 | 66 | 82 | ||
6863 | 6 | 1170 | 2268 | 8 | 100 | |
28 | 944 | 839 | 16 | 98 |
Values are the serum dilution at which relative luminescence units (RLUs) were reduced 50% compared to virus control wells (no test sample).