Table 4. H2d-Dextramer Staining and Intracellular Cytokine Staining of CD8+ T Cells from Immunized Mice.
Dextramer Staining | ICSa for IFN-γ | |||||||||
Group | Immunization | Mouse number | Experiment #1c | Experiment #2c | Mean | SDb | Experiment #1c | Experiment #2c | Mean | SDb |
9 | DNA | 1 | 0.2 | – | 0.77 | 0.67 | 1.3 | – | 1.53 | 0.40 |
2 | 0.6 | – | 2.0 | – | ||||||
3 | 1.5 | – | 1.3 | – | ||||||
10 | E2 | 4 | 0.2 | 0.2 | 0.62 | 0.46 | 1.1 | 1.7 | 2.32 | 1.21 |
LPS-positive | 5 | 0.7 | 1.3 | 4.6 | 1.9 | |||||
6 | 0.3 | 1.0 | 2.5 | 2.1 | ||||||
11 | DNA+E2 | 7 | 6.6 | 4.5 | 4.35 | 2.36 | 6.1 | 3.8 | 5.06 | 2.06 |
LPS-positive | 8 | 4.0 | 2.2 | 5.6 | 3.8 | |||||
9 | 7.4 | 1.4 | 8.4 | 2.7 | ||||||
12 | DNA+E2 | 10 | – | 7.0 | 7.43 | 5.26 | – | 4.4 | 5.56 | 2.46 |
LPS-free | 11 | – | 12.9 | – | 8.4 | |||||
12 | – | 2.4 | – | 3.9 | ||||||
13 | Unimmunized | 13 | 0.2 | 0.2 | 0.23 | 0.16 | 0.7 | 1.8 | 1.13 | 0.37 |
14 | 0.3 | 0.0 | 0.9 | 1.2 | ||||||
15 | 0.2 | 0.5 | 1.1 | 1.1 |
ICS, intracellular cytokine staining;
SD, standard deviation;
Values are expressed as percentages (%) minus the average percentage of the cells with medium alone; LPS, lipopolysaccharide; –, not done.