Table 4. H2^d-Dextramer Staining and Intracellular Cytokine Staining of CD8⁺ T Cells from Immunized Mice.

			Dextramer Staining		ICSa for IFN-γ
Group	Immunization	Mouse number	Experiment #1c	Experiment #2c	Mean	SDb	Experiment #1c	Experiment #2c	Mean	SDb
9	DNA	1	0.2	–	0.77	0.67	1.3	–	1.53	0.40
		2	0.6	–			2.0	–
		3	1.5	–			1.3	–
10	E2	4	0.2	0.2	0.62	0.46	1.1	1.7	2.32	1.21
	LPS-positive	5	0.7	1.3			4.6	1.9
		6	0.3	1.0			2.5	2.1
11	DNA+E2	7	6.6	4.5	4.35	2.36	6.1	3.8	5.06	2.06
	LPS-positive	8	4.0	2.2			5.6	3.8
		9	7.4	1.4			8.4	2.7
12	DNA+E2	10	–	7.0	7.43	5.26	–	4.4	5.56	2.46
	LPS-free	11	–	12.9			–	8.4
		12	–	2.4			–	3.9
13	Unimmunized	13	0.2	0.2	0.23	0.16	0.7	1.8	1.13	0.37
		14	0.3	0.0			0.9	1.2
		15	0.2	0.5			1.1	1.1

^aICS, intracellular cytokine staining;

^bSD, standard deviation;

^cValues are expressed as percentages (%) minus the average percentage of the cells with medium alone; LPS, lipopolysaccharide; –, not done.